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KMID : 0861020060210020135
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Korea Journal of Herbology
2006 Volume.21 No. 2 p.135 ~ p.142
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Inhibition of gene expression and production of iNOS and $TNF-{\alpha}$ in LPS-stimulated microglia by On-Bi-Tang
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Son Hye-Young
Kim Sang-Woon
Jung Sun-Ju
Jung Hyo-Won
Yoon Cheol-Ho
Park Yong-Ki
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Abstract
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Objectives: On-Bi-Tang(OB) has been prescribed Chinese traditional medicine for the treatment of inflammatory disease such as chronic renal failure. In this study, we investigated the anti-inflammatory effect of OB extract in the BV2 murine microglial cells.
Methods: After the water extract of OB was treated in BV2 cells, murine microglial line, the cell viability was measured by MTT assay. The production of nitric oxide (NO) and $TNF-{\alpha}$ was determined based on Griess reagent and enzyme linked immunosorbant assay (ELISA). mRNA expression of inducible nitric oxide synthase (iNOS) and $TNF-{\alpha}$ was analyzed by RT-PCR.
Results:OB extract significantly inhibited the LPS-induced production of NO and TNF-a in BV2 cells. OB extract also suppressed the mRNA expression of iNOS and $TNF-{\alpha}$ in BV2 cells activated with LPS.
Conclusions:These data suggests that OB extract may have the anti-inflammatory effect through the modulation of NO production and inflammatory cytokine such as $TNF-{\alpha}$.
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KEYWORD
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Anti-inflammation, BV2(microglial cell), iNOS, NO, On-Bi-Tang, $TNF-{\alpha}$
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